The goal of the Cre Driver Network is to provide the neuroscience community with mouse strains that are suitable for tissue- and time-specific perturbation of gene function in the nervous system.
|A Cre driver mouse brain with tamoxifen-inducible reporter gene expression in various brain regions.|
Using a knockout mutation to eliminate a gene’s function is a useful way to study the gene’s role in development, but it can lead to lethal or otherwise harmful effects that preclude studying the gene’s role in later stages of the mouse lifespan.
This project supports the generation of mice in which the expression of Cre recombinase, a DNA exchange enzyme, can be controlled temporally and spatially. These Cre “driver” lines can be used to generate conditional mutations that are activated in distinct cell types, tissues or time points, or inducible mutations that are activated through administration of a drug.
More than 100 Cre driver lines have now been created and characterized, targeting selected neuronal populations in the brain. Data and images detailing the Cre recombinase expression profile for each line are available. The Mice are available through the Mutant Mouse Regional Resource Center (MMRRC) at the University of Missouri or through The Jackson Laboratory’s Cre Repository for a nominal fee.