NIH Blueprint for Neuroscience Research

Gene & Protein Expression

 

Microarray Facilities

• Supports gene expression profiling of the nervous system using microarray technology. Provides reagents, services, and training to the neuroscience community on a fee-for-service basis.

• Samples from multiple tissues across the rodent life span are provided on a single slide.

Gene Expression Databases

• An ongoing contract to map gene expression in the mouse nervous system by generating mice whose endogenous genes have been replaced with eGFP reporter genes.
• All transgenic mice are deposited into NCRR's Mutant Mouse Regional Resource Centers.
• Related sites: NCBI GENSAT Database, St. Jude Brain Gene Expression Map

• The Allen Institute for Brain Science (Seattle, Washington) is under contract to conduct a high throughput analysis of gene expression in the developing Rhesus macaque brain.  Under a subcontract, the University of California Davis is collecting and preparing Rhesus macaque brain tissue for gene expression analysis.
• A publicly accessible gene expression map of the macaque brain is being released in stages.  This project will serve as an important link between efforts to map gene activity in the mouse brain and similar efforts to map the human brain.

• A project that uses Massively Parallel Signature Sequencing (MPSS) to map the gender-specific expression of mRNA transcripts in a variety of adult mouse tissues.

Antibodies

• Generates low-cost antibodies for the study of proteins found in mammalian brain.